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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: Hepatology. 2010 May;51(5):1692–1701. doi: 10.1002/hep.23501

Figure 6. Death ligands in hepatocytes and NPC fractions (A) and death receptors in hepatocytes (B) during hepatic I/R injury.

Figure 6

(A) WT (solid bars) and KO (open bars) liver grafts were obtained at 6 hours after LTx into WT, and hepatocyte and NPC fractions were isolated for RT-PCR analysis to determine mRNA expression of death ligands. Data were shown as fold increases over WT naïve hepatocytes or NPC (gray bars), respectively (n=3 for each group). The elevation of TRAIL and FasL mRNA levels during I/R were mainly seen in hepatocytes. TRAIL mRNA was completely inhibited in IRF-1 KO hepatocytes. FasL mRNA levels tended to be lower in the IRF-1 KO hepatocytes. (* p<0.05 vs. naïve WT, † p<0.05 vs. WT→ WT LTx)

(B) Death receptor mRNA expression in hepatocyte fraction was analyzed using WT (solid bars) and KO (open bars) liver grafts at 6 hours after LTx. Data were shown as fold increases over WT naïve hepatocytes (gray bars), (n=3 for each group). DR5 and Fas were upregulated in hepatocytes during I/R, and were significantly inhibited in hepatocytes obtained from KO→WT LTx. (* p<0.05 vs. naïve WT, † p<0.05 vs. WT→WT LTx)