Table 1.
Data collection and refinement statistics
| Dcn1P-Cdc53WHB (Form 1) 3O2P.pdb |
Dcn1P-Cdc53WHB (Form 2) 3O6B.pdb |
Ubc12 3O2U.pdb |
|
|---|---|---|---|
| Data Collection | |||
| Beamline | NECAT (ID-24-E APS) |
NECAT (ID-24-C APS) |
SERCAT (BM-22 APS) |
| Space group | P61 | P61 | P212121 |
| Cell dimensions | |||
| a, b, c (Å) | 57.157, 57.157, 177.725 |
123.914, 123.914, 192.558 |
56.451, 35.938, 87.035 |
| α, β, γ (°) | 90, 90, 120 | 90, 90, 120 | 90, 90.645, 90 |
| Resolution (Å) | 2.23 | 3.1 | 2.0 |
| Rmerge | 6.7 (20.5) | 9.0 (61.9) | 9.2 (49.4) |
| I/σI | 27.4 (11.75) | 22.6 (3.2) | 20.7 (2.5) |
| Completeness (%) | 99.9 (99.9) | 99.1 (99.6) | 98.5 (95.3) |
| Refinement | |||
| Resolution (Å) | 33.0–2.23 | 40.0–3.1 | 25.9–2.0 |
| No. reflections for refinement |
15730 | 28535 | 23515 |
| Rwork/Rfree | 16.9/22.6 | 25.8/30.8 | 18.5 / 23.3 |
| R.m.s deviations | |||
| Bond lengths (Å) | 0.007 | 0.011 | 0.007 |
| Bond angles (°) | 0.95 | 1.7 | 1.16 |
|
Ramachandran plot statistics |
|||
| Most favored regions | 96.8 | 91.8 | 97.0 |
| Additional allowed regions | 2.8 | 6.8 | 2.5 |
| Disallowed regions | 0.4 | 1.4 | 0.5 |
Data for highest resolution shell is shown in parentheses. Rwork = ∑|Fo-Fc|/∑FoRfree is the cross-validation of R-factor, with 5–10% of the total reflections omitted in model refinement.