Figure 7.

Increased cigarette smoke (CS)–induced activation of bronchoalveolar lavage (BAL) macrophages in aged mice. The macrophage surface marker F4/80 was used to identify BAL macrophages and nitric oxide synthase was used as a marker of macrophage activation. (A) Representative images of F4/80 positive BAL macrophages from 2- and 26-month-old mice exposed to air or CS for 5 days. (B) Fluorescent quantification of nitric oxide synthase expression in 2-, 13-, 19-, and 26-month-old mice exposed to air or 5-day CS. Data represented as mean ± SEM with *P < 0.05, **P < 0.01, ***P < 0.001 compared between air and CS; n = 4–6.