Figure 7.

Examples of transfected catecholaminergic and noncatecholaminergic Phox2b-positive neurons in the rostral ventrolateral medulla (RVLM). Left column illustrates the region immediately caudal to the facial motor nucleus. Almost all transfected cells (mCherry positive, red) have a Phox2b-immunoreactive nucleus (green) and most (small white arrows) contain tyrosine hydroxylase (TH, blue). The large white arrow points to a Phox2b-positive neuron that is transfected (mCherry positive) but does not contain TH. Right column illustrates the region immediately under the caudal end of the facial motor nucleus (the RTN). All transfected neurons in this region have a Phox2b-positive nucleus but they are devoid of TH (ccRTN neurons, white arrowheads). Rat brain tissue was cut into 30-μm sections through the RVLM and processed for immunohistochemistry as described in the Methods section. The scale bar at lower right (50 μm) applies to all panels.