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Lung sections stained immunohistochemically for (A–C) apelin or (D–F) APJ after early concurrent treatment on Day 10 in (A, D) room air and age-matched O2-exposed pups daily injected with (B, E) saline or (C, F) apelin. (G) APJ mRNA expression by in situ hybridization in a room air–exposed pup on Day 10. Arrows in G indicate APJ mRNA expression in alveolar capillaries and epithelial type 2 cells. a = alveolus; b = bronchus; bv = blood vessel.
