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. Author manuscript; available in PMC: 2010 Dec 13.
Published in final edited form as: Integr Biol (Camb). 2010 Aug 2;2(11-12):639–647. doi: 10.1039/c0ib00011f

Fig. 1. Chemotaxis assay for neutrophil migration and directional decision making in bifurcating channels.

Fig. 1

(a) Overall image of the microfluidic device. Buffer and chemokine were introduced from the left and split symmetrically on the two sides of the array. This created the conditions for an exclusively diffusion-driven gradient along the channels in array. A detail of the channel array, imaged using scanning electron microscope is presented. (b) Inside asymmetric mazes, human neutrophils chose the shorter path towards the source of fMLP 100 nM in the top channel in 90% of the observations (red outline). (c) In control experiments, human neutrophils showed no significant preference for path in symmetric mazes. The position of the neutrophils in the images is indicated by the red arrow, and displayed at 100 or 200 s intervals.