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. Author manuscript; available in PMC: 2010 Dec 13.
Published in final edited form as: Acta Neuropathol. 2008 Mar 20;116(1):79–86. doi: 10.1007/s00401-008-0359-1

Figure 1. Immunohistochemical and In Situ Hybridization (ISH) staining for HCMV.

Figure 1

(A) Immunohistochemical stain of a GBM section without an antibody (negative control). (B) Immunohistochemical stain of a GBM section with an antibody against actin (positive control). (C) Immunohistochemical stain of a GBM section with an antibody against the HCMV IE1-72 protein. (D) ISH stain of a GBM section with a probe against black beetle virus DNA (negative control). (E) ISH stain of a GBM section with an Alu DNA probe (positive control). (F) ISH stain of a GBM section with an HCMV DNA probe. Panels A, B, and C represent immunohistochemical staining of serial sections from the same GBM specimen, and panels D, E, and F represent ISH staining of serial sections from the same GBM specimen. We acquired images of stained GBM sections using a microscope with a Nikon Plan Fluor 20×/0.50 (Panels A-F) (Nikon, Melville, NY, USA).