Figure 5.

A comparison of whole cell lysates and proteolytically-cleaved cell surface antigens in mediating the lysis of breast adenocarcinoma cells in cytotoxicity assays. Target adenocarcinoma cells, MCF-7, were incubated with effector CTLs at a ratio of 4:1. On the 3^rd day, target cells were carefully washed and imaged using an inverted phase contrast microscope (scale bar: 50 µm). (a) Initial cell culture of MCF-7 cells. (b) Control MCF-7 cells grown alone. (c) MCF-7 cells incubated with CTLs that had been stimulated with dendritic cells loaded with whole cell lysates. (d) MCF-7 incubated with CTLs that had been stimulated with dendritic cells loaded with cell surface antigens. Cell surface antigens show the same ability to induce cytotoxic activity in CTLs as whole cell lysates. Adapted from Balashova et al. ⁶⁴.