Skip to main content
. Author manuscript; available in PMC: 2010 Dec 13.
Published in final edited form as: J Biol Chem. 2007 May 22;282(29):21237–21243. doi: 10.1074/jbc.M701341200

FIGURE 3. Effect of ox-PAPC on p38-MAPK and ERK.

FIGURE 3

A, activation of p38-MAPK in response to BMP-2 and ox-PAPC. Western analysis of phosphorylated p38-MAPK from cultures treated with vehicle, BMP-2 (100 ng/ml), and/or ox-PAPC (10μg/ml) for 30 min (left panel)or3 h(right panel). Total p38-MAPK was used as a loading control. B, alkaline phosphatase activity in response to BMP-2, ox-PAPC, and SB203580. Cells were pretreated for 30 min with SB203580 (10 μm), followed by treatment for 2 days with vehicle, BMP-2 (100 ng/ml), ox-PAPC (10 μg/ml), and/or SB203580 (10 μm) as indicated. *, p < 0.0001. C, activation of SMADs in response to BMP-2 and ox-PAPC. Western analysis of phosphorylated SMAD 1/5/8 in cells treated for 1.5 h with vehicle, BMP-2 (100 ng/ml), ox-PAPC (30 μg/ml), or BMP-2/ox-PAPC. Total SMAD 1/5/8 was used as a loading control. D, immunofluorescent (fluorescein isothiocyanate) staining of phosphorylated SMAD 1/5/8 (green; left panels) in cells treated for 1.5 h with vehicle (a), ox-PAPC (10 μg/ml) (b), BMP-2 (50 ng/ml) (c), or BMP-2 and ox-PAPC (d). 4′,6-Diamidino-2-phenylindole staining (blue; right panels) was used to visualize cell nuclei (magnification ×400).