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. Author manuscript; available in PMC: 2010 Dec 13.
Published in final edited form as: Proteomics. 2009 Nov;9(22):5029–5045. doi: 10.1002/pmic.200900196

Fig. 4.

Fig. 4

Proteins derived from the insoluble fraction of a strain Sd1617 cell lysate separated in 2D gels in the pH range 4–7. Cells were isolated from the large bowel of infected piglet #1. The insoluble fraction was extracted with 2.5 M NaBr (hs-MBR fraction, left), followed by solubilization with 8 M urea, 2 M thiourea and 1% amidosulfobetaine-14 (usb-MBR fraction, right). Experimental conditions for 2D gel electrophoresis and staining are described in the Methods. Several proteins are listed in Table 1 with further descriptions. Integral OM proteins (e.g. OmpA and OmpX) were enriched in the usb-MBR fraction. Proteins responding to various stresses (e.g. AdiA, GadB, GroEL, DnaK, HtpG and ClpB) were relatively abundant in both fractions.