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. Author manuscript; available in PMC: 2010 Dec 13.
Published in final edited form as: Proteomics. 2009 Nov;9(22):5029–5045. doi: 10.1002/pmic.200900196

Fig. 6.

Fig. 6

S. dysenteriae serotype 1 antigens detected with a piglet antiserum in a 2D western blot. Left: cell lysate; right: usb-MBR fraction. Matching CBB-stained 2D gels were used to identify the spots by MS. Thorough validation was required to designate a protein confidently as an antigen: (1) highest LC-MS/MS score from at least two gel spots matching the western blot signal; (2) same spot shape in 2D gel and western blot; (3) availability of MALDI-TOF data (IpaB and VirB were the exceptions); (4) cross-validation of data via SDS-PAGE western blotting after fractionation of a cell lysate by anion exchange chromatography (data not shown here).