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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: Maturitas. 2010 May 7;66(3):315–322. doi: 10.1016/j.maturitas.2010.04.001

Figure 2.

Figure 2

The ER antagonist ICI 182780 specifically blocks the induction of luciferase activity by JP-M through ERα in MCF-7 cells transiently transfected with a pERE-luciferase plasmid. Cells were treated with 10 nM E2 or 10 nM E2 + 1 µM ICI 182780 or 20 µg/ml JP-M or 20 µg/ml JP-M + 1 µM ICI 182780 to assess induction. Relative luciferase units were measured by luminometer. Results are expressed as fold induction above control (vehicle solvent). Abbreviations: E2 = 17β-estradiol; JP-M = Justicia pectoralis; ICI = ICI 182,780. Data represent the mean ± SD of three separate experiments performed in triplicate.