Figure 3.
Results of RT-PCR analysis of endogenous estrogen responsive genes in MCF-7 cells after treatment with plant extracts (20 µg/ml). Data is represented as fold increase in mRNA above control for (3) pS2, (4) PR, (5) PTGES. Extracts are abbreviated: JP-M = Justicia pectoralis. MCF-7 cells were treated with the JP-M extract (20 µg/ml) + E2 (10 nM) to assess synergistic or antagonistic effects. Data represent the mean ± SD of three separate experiments performed in triplicate.