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. Author manuscript; available in PMC: 2011 Dec 7.
Published in final edited form as: Biochemistry. 2010 Nov 12;49(48):10287–10297. doi: 10.1021/bi100500m

Figure 3. Ca2+ -dependent changes in amide chemical shifts of C-CaM show strong cooperativity.

Figure 3

Resonance of Thr117 in the linker region of C-CaM exhibited two-state slow exchange at various molar ratio of Ca2+ added into C-CaM, indicating highly cooperative Ca2+ binding to sites III and IV. (A) [Ca2+]/[C-CaM]=0; (B) [Ca2+]/[C-CaM]=0.3; (C) [Ca2+]/[C-CaM]=0.6; (D) [Ca2+]/[C-CaM]=2.0.