. Author manuscript; available in PMC: 2011 Dec 7.

Published in final edited form as: Biochemistry. 2010 Nov 12;49(48):10287–10297. doi: 10.1021/bi100500m

Table 2.

Summary of Residues that Undergo Conformational Exchange on the μs-ms Timescale

Protein	R₂/R₁		R₂R₁		Reduced Spectral Density Mapping		Exchange Broadening
Apo C-CaM	Glu87 Arg90 Phe92 Ile100	Met109 Thr110 Asp131 Glu139	Glu87 Arg90 Phe92 Ile100	Met109 Thr110 Asp131 Glu139	Glu87 Arg90 Phe92 Ile100	Met109 Thr110 Asp131 Glu139
Apo C-CaM +PEP-19	Ala102 Thr110 Glu114 Ile125 Glu127	Ala128 Gly132 Val136 Glu139	Ala102 Thr110 Glu114 Asp122 Ile125 Arg126	Glu127 Ala128 Gly132 Val136 Glu139	Ala102 Thr110 Glu114 Ile125 Glu127	Ala128 Gly132 Val136 Glu139	Asp131 Gly134
Ca²⁺-C-CaM	Asp93 Glu104 His107 Met109	Leu112 Gly113 Ile130	Ser101 Glu104 His107	Met109 Leu112	Glu104 His107	Leu112 Met 109
Ca²⁺-C-CaM +PEP-19	Asp93 Met109 Leu116	Thr117 Val121 Thr146	Asp93 Met109 Leu116 Thr117	Val121 Gln143 Thr146	Asp93 Met109 Leu116	Thr117 Val121 Thr146	Thr 110 Asn111 Gly 113	Glu114 Lys115

Residues exhibiting conformational exchange on the μs-ms timescale by all criteria are shown in bold.