Fig. 6.

DNA-SCARS are caused by multiple senescence inducers and in several cell types. (A) HCA2 cells were stained for 53BP1 foci (panel 1) and PML NBs (panel 2). Colocalization is shown in yellow (panel 3). Untreated cells were fixed at PD23 (‘Control’), and PD25 cells were infected with a lentivirus encoding either oncogenic Rasv12 (Ras) or a dominant-negative TIN2 protein (DN-Tin2). After a recovery period of 3–4 days, the cells were fixed and stained. Cells were also treated for 1 hour with 100 μM H₂O₂ (H₂O₂); 5 days later, they were fixed and stained. (B) Primary HMECs, HUVECs and HAECs were fixed and stained as in A 8 or 10 days, as indicated, after receiving 10 Gy irradiation.