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. 2010 Dec 13;5(12):e14291. doi: 10.1371/journal.pone.0014291

Figure 4. UEV-1 is required for motoneuron synaptic bouton differentiation.

Figure 4

(A–D) Fluorescence from animals transgenic for Punc-25::snb-1::gfp (synaptobrevin). (A) Presynaptic boutons labeled with SNB-1::GFP are distinct along the dorsal and ventral cords of wild-type animals. Such boutons are missing, irregular in shape and size, and/or abnormally spaced in (C) uev-1(od10) mutants, a phenotype reminiscent of that observed in (B) rpm-1 mutants. (D) Mutations in pmk-3 do not alter the uev-1 phenotype. (E) The total number of dorsal cord SNB-1::GFP puncta is plotted for the indicated genotypes. (F) GLR-1::GFP accumulation in accretions in uev-1 pmk-3 double mutants is similar to that observed in uev-1(od10) single mutants. Bar, 5 microns. Error bars are SEM. N = 20–30 animals for each genotype. ***P<0.001 by ANOVA with Bonferroni multiple comparison tests.