Figure 4. T helper cell cytokine profiles.

A) One million PBMC were stimulated for 5 hours with PMA and calcium ionomycin in the presence of monensin, and subsequently stained for flow cytometric analysis. In the lymphogate, CD3⁺CD8⁻ cells were assessed for the proportions of IFN-γ⁺, IL-4⁺, IL-17⁺ and IL-10⁺ cells. CD69 was included to evaluate activation. B) The proportions of IL-10⁺ CD4⁺ T cells before and after supplementation. C) The ratio between Th1 (IFN-γ⁺) and Th2 (IL-4⁺) cells before and after supplementation. Significance was assessed with the Wilcoxon signed ranks comparison test.