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. 2010 Dec 13;5(12):e14304. doi: 10.1371/journal.pone.0014304

Figure 1. Expression of CD146, CXCL12 and Angiopoietin 1 in stromal cells and primary long term cultures (LTCs).

Figure 1

A: FACS analysis of stromal cell lines HS5 and HS27a for CD146 (MCAM) along with isotype controls. B: FACS analysis of a primary LTC at first passage for CD146 along with isotype control. The primary LTCs were sorted to CD146 hi and CD146 lo populations by FACS-aided flow-sorting. Approximate gates for hi and lo populations are also indicated. C: CXCL12 levels of 9 LTCs sorted to CD146hi and CD146lo and quantitated by RT-PCR (relative to beta-actin, multiplied by a factor of 1000). Paired samples from the same culture are connected by a line. D: Angiopoietin-1 levels of the same 9 LTCs, also quantitated by RT-PCR (relative to beta-actin, multiplied by a factor of 10000). Experiment in panel A was confirmed in three independent experiments, Experiments in panels B, C and D were confirmed in 9 independent samples. Statistical analysis of the datasets in Panels C and D by Wilcoxon's matched pair test revealed a p-value of <0.01 in both cases.