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. 2010 Dec 13;5(12):e15278. doi: 10.1371/journal.pone.0015278

Figure 3. DPE-L1 and DPE-L2 functions are not additive.

Figure 3

A) Schematic illustration of DPE-L1, DPE-L2, GLE, DPE-L1/2 and GLE/DPE-L1/2 mutants. Double and triple mutations of DPE-L1, DPE-L2, and GLE were designed to encompass the entire region of the each element identified by the scanning mutations. Thus, the mutations in the DPE-L1/2 double promoter mutant extended across +13 to 18 bp and +27 to+30 bp and the GLE/DPE-L1/2 triple promoter mutant included mutations at +4 to +9 bp, +13 to 18 bp and +27 to+30 bp. The single GLE and DPE-L1 and DPE-L2 mutations were the same as shown in Figure 2. B) The promoter activity of DPE1/2 is indistinguishable from that of either of single DPE mutants. The two single mutant constructs and the double DPE mutant construct were transfected into HeLa cells or HeLa/CIITA cells and the promoter activity was determined relative to wild type promoter (WT+32). C) GLE functions independently of DPE-Ls in basal transcription, but not in activated transcription. The double mutant DPE-L1/2 and the triple mutant GLE/DPE-L1/2 constructs were transfected into HeLa cells or HeLa/CIITA cells and their activities were compared.