Figure 7. C57BL/6 mice pre-treated with the GalR1 antagonist, galantide, show increased susceptibility to the neurotoxic effects of kainate.

Corresponding low-power and high-power photomicrographs of NeuN- stained horizontal sections of the hippocampus showing differential cell loss 7 days after kainate-induced SE (vehicle), and in a representative mouse pre-treated with the GalR1 antagonist, galantide. Note that while hippocampal cell death is essentially non-existent following kainate-induced status epilepticus (Vehicle) to excitotoxin cell death-resistant mice (C57BL/6), a massive loss of neurons, as evidenced by loss of immunostaining in the hilar, CA3, and CA1 fields of the hippocampus, was induced by pre-treatment with galantide prior to KA-induced SE. CA1 and CA3 denote the hippocampal subfields; H, dentate hilus. Scale bars: top panels, 750 µm; bottom panels, 100 µm.