Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Dec 13;5(12):e15251. doi: 10.1371/journal.pone.0015251

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

PMC Copyright notice

Note the gross reduction of TH immunoreactivity in the SN at the site of injection (B; yellow arrowhead) versus the non-injected side (A). Similar loss of TH staining is seen in the striatum ipsilateral to the injection (D, arrows) versus that on the non-injected side (C, arrows), suggesting disruption of nigral dopaminergic terminals. The area just lateral to the anterior commissure (D, yellow arrowhead) however was always densely labeled (see text). Densitometry of immunostaining of striatal TH (E) showed significant differences (p<0.001) of the whole striatum contralateral and ipsilateral to DOPAL injections. Spot density measurements of ventrolateral parts of the striatum (D, red circles), however, showed an 80% loss of immunoreactivity ipsilateral to the injection. Intensely stained neurons with antibodies against tyrosine hydroxylase (F, yellow arrowheads) were sometimes seen in the SNpc of control brains surrounded by numerous neurons stained only for Nissl (F, black arrows), suggesting that counting only TH-immunostained neurons may be problematic. Abbreviations: ac, anterior commissure; SNpc, pars compacta of substantia nigra; SNpr, pars reticulata of substantia nigra. *** p<0.001.