Figure 1.

Schematic representation of the regulation of AMT1;1 trimer. The homotrimer (blue) is represented with the C-terminus (brown line) connecting the neighboring subunit. In absence of phosphorylation of T460, the pores are in open conformation, enabling ammonium to enter the cell. A single phosphorylation event in the C-terminus of one the monomers (red phosphate), induces a conformational change which is sufficient for cooperative closure of the trimer. Multiple phosphorylation events could potentially maintain the complex closed.