Comparison of Mirus Label IT and 5′ end labeled amplicons. Fifty nanograms of the labeled template was hybridized with different sets of K. brevis (Pkb, Pkb2, PKB658, Pkb2lna, PKB658lna) and K. mikimotoi probes (Pkmiklna and Pkmiklna2). Hybridization assay was undertaken at 50°C for 1.5 h. The error bars represent the standard deviation. The samples were run in duplicate, and the experiments were run twice.