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. Author manuscript; available in PMC: 2011 Dec 15.
Published in final edited form as: Bioorg Med Chem Lett. 2010 Oct 21;20(24):7323–7326. doi: 10.1016/j.bmcl.2010.10.060

Figure 4. Colony Formation Assay.

Figure 4

Colony formation assays were used to assess the potency of 6 drug analogs.9 HUVECs growing exponentially in T25 flasks (n = 4) were exposed to DMSO/analog for 6 hours at 37 °C. Analog was then washed off, and cells were incubated in fresh medium for 14 days. Viable cells formed colonies that were stained with crystal violet and counted. The plating efficiency was defined as the ratio of the number of colonies counted divided by the initial number of cells exposed to vehicle control (DMSO) for 6 h. Vehicle treatment (6 h) was not toxic to cells, as survival was not significantly different from plating efficiency measured in the absence of vehicle (p>0.05 Student’s t-test) and was arbitrarily set at 1.0. All analogs were tested at 10 µM. The percentage of surviving cells following exposure to analog alone was 75% or greater.