| Macromolecule details |
|
| Database code |
PDB code 3nad
|
| Component molecules |
Phenolic acid decarboxylase (PAD; EC 4.1.1.) |
| Macromolecular assembly |
PAD is a dimer contained within the asymmetric unit |
| Mass (Da) |
19082 (method: mass spectrometry) |
| Source organism |
B. pumilus strain UI-670 |
| Macromolecule production |
|
| Cloning |
PCR-amplified from genomic DNA, cloned into the vector pKK223-3 and expressed in E. coli JM109 |
| Crystallization and crystal data |
|
| Crystallization method |
Vapour diffusion |
| Temperature (K) |
293 |
| Apparatus |
Linbro crystallization plates |
| Atmosphere |
Room air |
| Crystal-growth time (d) |
23 |
| Additional details |
Crystals were grown by mixing 1l protein with 1l reservoir solution and equilibrating over 0.5ml reservoir solution |
| Crystallization solutions |
|
| Macromolecule |
1l PAD (21.3mgml1), 20mM TrisHCl pH 7.2, 150mM sodium chloride pH 7.2 |
| Precipitant |
1.0l 0.2M disodium tartrate, 0.1M trisodium citrate pH 5.6, 0.5M ammonium sulfate pH 5.6 |
| Crystal data |
|
| Crystal colour |
Clear light |
| Crystal shape |
Plates or prisms |
| Crystal dimensions (mm) |
0.2 0.05 0.1 |
| Matthews coefficient V
M (3Da1) |
2.69 |
| Solvent content (%) |
53.9 |
| Unit-cell data |
|
| Crystal system, space group |
Orthorhombic, P21212 |
| Unit-cell parameters (, ) |
a = 92.11, b = 109.96, c = 45.43, = = = 90 |
| No. of molecules in unit cell Z
|
8 |
