Table 4. Purification of recombinant B. pumilus PAD expressed in E. coli .
n.a., not applicable.
| Purification step | Enzyme composition | Total protein (mg) | Total activity† (U) | Specific activity (Umg1) | Purification (fold) | Yield (%) |
|---|---|---|---|---|---|---|
| Crude extract | n.a. | 945 | 6610 | 7 | 1 | 100 |
| DEAE Sepharose | n.a. | 144 | 8400 | 58 | 8 | 127 |
| Butyl-S Sepharose | 2 | 82 | 7950 | 97 | 14 | 120 |
| 4 | 3 | 582 | 194 | 28 | 9 |
†
The lower total units of activity in the crude extract fraction compared with subsequent purification steps is possibly the result of inhibition by some component of the culture medium.