Table 2. Profiling of proteolytic activities in IRS-2 protein preparation.
Substrate | Protease type (catalytic class) | Proteolytic activity | Inhibitor sensitivity |
---|---|---|---|
Z-Phe-Arg-AMC | Papain-like (cysteine) | Yes | E64 |
Trypsin-like (serine) | Pefabloc | ||
MeoSuc-Ala-Ala-Pro-Val-AMC | Elastase-like (serine) | No | |
Z-Gly-Gly-Leu-AMC | Chymotrypsin-like (serine) | Yes | Pefabloc |
Suc-Ala-Ala-Pro-Phe-AMC | Chymotrypsin-like (serine) | No | |
Suc-Leu-Tyr-AMC | Calpain-like (cysteine) | Yes | E64 |
Chymotrypsin-like (serine) | Pefabloc |