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. 2010 Oct 29;66(Pt 11):1536–1540. doi: 10.1107/S1744309110038698

Figure 2.

Figure 2

Resonance Raman spectra of crystals of Hb1Em in 100 mM Tris–HCl buffer pH 8.0 at room temperature in the carbomonoxy (CO), deoxygenated (Deoxy) and ferric (Met) forms for Hexa (a) and Ortho (b) crystals. The Ortho deoxygenated form (b) converts quickly into haemochrome (Haemo) after 10 min laser exposure. Excitation wavelength, 514.5 nm; laser power at the sample 2 mW for the ferric and deoxy forms and 0.1 mW for the carbomonoxy form. All spectra were an average of at least six spectra with 2 min integration time.