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Mitochondrial DNA detected in the supernatant fluid from NT-stimulated LAD2 cells. LAD2 cells were stimulated with NT (1, 5, 10 μM) for 1 hr. Mitochondrial specific DNA mt-7S and mt-CytB were detected and quantified by Real time PCR using Taqman assay (Applied Biosystems, CA). Genomic DNA GAPDH was undetectable, excluding the possibility of cell contamination (*p < 0.01, **p < 0.0001).
