Table 2.
Strains | Relevant characteristic | Reference |
---|---|---|
S. aureus 8325-4 | wild type | [27] |
8325-4 hssR::bursa | resistant mutant, bursa insertion | This work |
8325-4 hssR | hssR mutation transduced from 8325-4 hssR::bursa | This work |
S. aureus 15981 | wild type | [34] |
S. aureus 15981ΔTCS15 | hssRS deletion | [18] |
8325-4 hssR::bursa/pRMC2-hssRS | Complementation of the transposon mutant | This work |
L. monocytogenes 4446 | wild type | [35] |
L. monocytogenes LO28 | wild type | [36] |
LO28 RR23 | rr23 mutant | [22] |
Plasmids | ||
pRMC2 | Tetracycline inducible expression vector | [37] |
pRMC2-hssRS | hssRS cloned into pRMC2, complementation plasmid | This work |