Figure 9.
Effect of choline-derived analogues on Ψm of T. brucei bloodstream forms. Effects on Ψm were measured by flow cytometric analysis of parasites loaded with 25 nM TMRE. Control (a) and treated cells [(d) 5 µM T1; (e) 5 µM T4; (f) 2 µM M38; (g) 5 µM G25; (h) 2 µM MS1] were harvested at (I) 8 and (II) 16 h and prepared for Ψm analysis as described in the Materials and methods section. Positive controls were performed with 100 nM valinomycin (b) and 10 µM troglitazone (c) to decrease and increase the Ψm, respectively.