Figure 2. Notch is not required for CBP expression.

(A) shows the location of four putative binding sites for Su(H) near the transcription unit of CBP. Boxes indicate exons and filled regions represent sequences that are translated. The position of each element is indicated relative to the start of transcription (+1, arrow). (B) is a western analysis of Notch and CBP protein levels under conditions in which Notch is silenced with RNAi by applying multiple heat shocks (see Materials and Methods). Two blots were made from each protein sample. One blot was incubated with Notch antibody (top row) and the other was incubated with CBP antibody (3^rd row). Both blots were cut and incubated separately with tubulin antibody to serve as a control for protein loading and transfer (2^nd and 4^th rows). (C–D) are confocal images of dissected brains from control (no Gal4, UAS-Ni) and Notch-silenced experimental animals (hsGal4; UAS-Ni). Both groups were heat-shocked using the same regimen as B, and both were stained with CBP antibody and imaged using the same conditions and microscope settings.