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. 2010 Dec 14;5(12):e14311. doi: 10.1371/journal.pone.0014311

Figure 6. Knockdown of endogenous Sp1 decreases mHtt-mediated REST activation.

Figure 6

NG108 cells were cotransfected with Nter-15Q or Nter-142Q vectors and Sp1 or/and Sp3 siRNA. After 6 days of neuronal differentiation, REST mRNA level was analyzed by quantitative RT-PCR. Each bar represents the mean values ± sd of at least 2 independent experiments performed in triplicate. ***P<0,001, Nter-15Q neuronal-like cells vs. Nter-142Q neuronal-like cells; ###P<0,001, Nter-15Q neuronal-like cells transfected with control siRNA vs Nter-15Q neuronal-like cells transfected with Sp3 siRNA or Sp1/Sp3 siRNA.