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. 2010 Dec 14;5(12):e14335. doi: 10.1371/journal.pone.0014335

Figure 2. LBH589 treatment leads to mitotic abnormalities and cytokinesis failures.

Figure 2

A549 cells were treated with vehicle (control) or 40 nM LBH589 for 24 hours. A, arrows show bi- or multinucleated cells with impaired cytokinesis in LBH589-treated NSCLC cells. B, cells were fixed and stained with DAPI or cleaved poly (ADP-ribose) polymerase (cPARP) antibody (×100 or ×400 magnification). C, Western blot analysis demonstrating that HDAC inhibition by LBH589 causes histone H3 phosphorylation (H3-P10), histone H4 acetylation (Acety-H4), and PARP cleavage (cPARP) in A549 cells treated with LBH589 for 24 hours. β-actin was used as loading control.