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. 2010 Dec 14;5(12):e15620. doi: 10.1371/journal.pone.0015620

Figure 2. RanBP2 depletion impairs biological activities of ectopically expressed Tat and Rev HIV proteins.

Figure 2

(A) Schematic diagram of Tat/Rev responsive SVGA-LTR-gag-GFP cells is shown. The cells were transfected with either 50 ng NL4-3 plasmid, or 50 ng NL4-3 (Rev-). Fluorescent images were captured 48 h post transfection. (B) SVGA-LTR-gag-GFP reporter cells were transfected either with RanBP2 siRNA, RanBP3 siRNA (negative control) or scrambled siRNA (control). 48 h later, the cells were then co-transfected with 50 ng pCMV-Tat and 200 ng pCMV-Rev. In parallel, Leptomycin B was used as positive control and was added 4 h after the transfection of reporter cells (pre-transfected with scrambled siRNA) with pCMV-Tat and pCMV-Rev. The images were captured and GFP positive cells were analyzed by flow cytometry 48 h post plasmid transfection.