Figure 4. RanBP2 depletion does not inhibit GFP expression in SVGA-GFP transgenic cells.

Stable SVGA-GFP cells were transfected overnight either with 100 nM RanBP2 siRNA, 200 nM RanBP3 siRNA (negative control), 100 nM scrambled siRNA (control), or 100 nM GFP siRNA as a positive control. The cells were then followed up for 5 days. On day 3 images were captured and the GFP positive cells were analyzed by flow cytometry.