Skip to main content
. 2010 Dec 14;5(12):e15620. doi: 10.1371/journal.pone.0015620

Figure 6. RanBP2 depletion inhibits HIV-1 replication and lentiviral vector gene expression.

Figure 6

(A) Magi cells were transfected either with RanBP2 siRNA, RanBP3 siRNA (negative control), TNPO3 siRNA (positive control) or scrambled siRNA (control). 48 h later, the cells were transduced with 200 ng/ml p24 equivalent VSV-NLENY1 (recombinant HIV virus containing YFP) for 16 h. The fluorescent images were taken 48 h post transduction followed by collection of the culture supernatants for p24 (ELISA). The YFP positive cells were analyzed by flow cytometry (p<0.01). (B) SVGA cells were transfected either with RanBP2 siRNA, RanBP3 siRNA or scrambled siRNA. 48 h later, the cells were transduced with 200 ng/ml p24 equivalent VSV-NLENY1 for 16 h. The fluorescent images were captured 48 h post transduction by digital camera (Nikon) and YFP positive cells were analyzed by flow cytometry (p<0.001). (C) SVGA-LTR-GFP reporter cells were transduced either with RanBP2 siRNA, RanBP3 siRNA (negative control), scramble siRNA or TNPO3 siRNA as a positive control. 48 hrs later, the cells were then transduced with 200 ng/ml p24 equivalent VSV-NL4-3. Images were captured 48 h after transduction and the GFP positive cells were analyzed by flow cytometry (p<0.01). (D) SVGA cells were transfected either with RanBP2 siRNA, RanBP3 siRNA, TNPO3 siRNA or scrambled siRNA. 48 h later, the cells were transduced with VSV-G pseudotyped lentiviral vector. The fluorescent images were captured 48 h after transduction and GFP positive cells were analyzed by flow cytometry (p<0.01).