Table 1. Clusters of ≥3 genes with statistically significant changes in expression common to Ea.hy926 endothelial cells infected with L. biflexa serovar Patoc and L. interrogans serovar Canicola.
| KEGG pathway1 | genes, L. biflexa | p value, L. biflexa 2 | genes, L. interrogans | p value, L. interrogans |
| Focal adhesion (FA) | 7 | 1.00e-6 | 7 | 7.94e-6 |
| Regulation of actin cytoskeleton (ACT) | 3 | 1.75e-2 | 4 | 6.59e-3 |
| Leukocyte transendothelial migration (TEM) | 2 | 3.83e-2 | 3 | 8.73e-3 |
| Phosphatidylinositol signaling system (PI) | 3 | 9.28e-4 | 2 | 2.78e-2 |
| ECM-receptor interaction (ECM) | 3 | 1.53e-3 | 2 | 3.82e-2 |
1
KEGG Pathways: http://www.genome.jp/kegg/pathway.html. The Table is arranged in order of descending p value for L. interrogans. The comparisons were made between cells infected with the indicated bacterial strain vs. uninfected controls at 1 hr. post infection. Similar trends were observed at the 3 hr. time point. Pathway abbreviations are provided for reference to Table 2. The same pathways also showed significant changes in expression, in similar patterns, in a second endothelial cell line, HMEC (data not shown).
2
P values considered to be statistically significant were <0.05 by the Hypergeometric test. ( http://bioinfo.vanderbilt.edu/webgestalt ).