Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Dec 13;191(6):1189–1203. doi: 10.1083/jcb.201005035

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2010 Nievergall et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 5. — PTP1B interacts with EphA3 at the cell surface. (A) EphA3 clustering by SA beads provokes tyrosine phosphorylation. COS7 cells, cotransfected with AP_N-EphA3 and BirA, were stimulated with SA Dynabeads. Tyrosine phosphorylation was evaluated by confocal microscopy of fixed and permeabilized cells stained with ^Cy3.5α-PY (PY72) antibodies. SA Dynabeads appear blue in the merged image; yellow arrows mark beads with α-PY association. (B) COS7 cells, cotransfected with GFP-PTP1B-[D-A], AP_N-EphA3, and TM-BirA (as indicated), were stimulated with SA Dynabeads. Cell surface EphA3 was labeled on intact cells with IIIA4 α-EphA3 mAb and detected with Alexa Fluor 546–labeled secondary antibodies by confocal microscopy. Yellow arrows mark SA Dynabeads where PTP1B/EphA3 colocalization is apparent. (C) GFP-PTP1B-[D-A] and EphA3-transfected COS7 cells were stimulated with Alexa Fluor 594–ephrinA5–Fc–coated Protein A Dynabeads, then fixed and analyzed by confocal microscopy. White dotted lines mark cell boundaries from bright-field images.