Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Dec 13;191(6):1141–1158. doi: 10.1083/jcb.201007152

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2010 Otera et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 5. — Mff-induced mitochondrial fission depends on Drp1. (A) FLAG-Mff was transfected in WT or Drp1^−/− MEFs. After 36 h, the cells were fixed and immunostained with anti-Tom70 antibodies (green) and anti-FLAG antibodies (red). (B) Percentage of cells with tubular and fragmented mitochondria. Three distinct fields for each 100 cells were counted. Data represent mean ± SD. (C) HeLa cells stably expressing Su9-DsRed were transfected with the indicated siRNA and further transfected with pMff-IRES-GFP-NLS. Live images were obtained by confocal microscopy. (D) HeLa cells in C were subjected to immunoblot analysis using the indicated antibodies with mitofilin as a loading control. Molecular mass is given in kilodaltons. (E) Percentages of cells (n = 100) with the indicated mitochondrial morphologies in mock- and Mff-transfected cells at 24 h after transfection. Data were obtained from three independent experiments and represent mean ± SD.