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. 2010 Dec 13;191(6):1141–1158. doi: 10.1083/jcb.201007152

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© 2010 Otera et al.

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Figure 6. — Depletion of Mff, but not hFis1, blocks Opa1 RNAi–induced extensive mitochondrial fragmentation and increased hypersensitivity to apoptosis. (A) HeLa cells stably expressing Su9-DsRed were transfected with the indicated siRNA. Live images were obtained by fluorescence microscopy (a–e magnified images are shown as insets in b and e). EM of these manipulated cells are shown in f–j. (B) Onionlike mitochondrial inner membrane structures in Opa1/Drp1 and Opa1/Mff double knockdown cells. (C) Percentages of cells with the indicated mitochondrial morphologies in various RNAi cells. Data were obtained from 100 cells in each of three independent experiments and represent mean ± SD. (D) HeLa cells were transfected with the indicated siRNA and then treated with actinomycin D in the presence of Z-VAD-FMK. Cells were fixed at the indicated time points, and cytochrome c release was detected by immunostaining. Data were obtained from 300 cells in each of three independent experiments and represent mean ± SD.