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. 2010 Dec 13;191(6):1113–1125. doi: 10.1083/jcb.201006121

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© 2010 Li et al.

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Figure 2. — Oxidative stress and apoptosis in ER-stressed macrophages are dependent on NOX2. (A and B) Macrophages were transfected with Nox2 siRNA and, after 72 h, were incubated without sterol (Con), under cholesterol-loading conditions (CHOL), or with 7-ketocholesterol (7KC). The cells were assayed for Nox2 mRNA by RT-QPCR after 8 h (A) and DCF fluorescence and annexin V staining after 30 h (B). (C) Macrophages from WT or Nox2^−/− mice were incubated for 14 h without sterol, under cholesterol-loading conditions, or with 7-ketocholesterol and then assayed for DCF and annexin V staining. (D) Macrophages from WT or Nox2^−/− mice were incubated under cholesterol-loading conditions for 24 h or with 7-ketocholesterol for 20 h and then assayed for apoptosis by TUNEL staining. Bars with the same symbols are not significantly different from each other, whereas bars with different symbols are significantly different from each other. scrRNA, scrambled RNA. n = 3 for each experimental group. Data are presented as means ± SEM.