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. 2010 Dec;60(6):427–435.

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Figure 4. — Alignment of a region of rpoB from Heyl, Jawetz, and Pasteurellaceae–other. Sequences were aligned by using Vector NTI software; isolates with identical 16S rRNA and rpoB genes have been omitted from the alignment. Green box, Heyl isolates characterized during the current study; black box, Jawetz isolates (characterized during the current study and AY31404), red boxes, 16S rRNA gene sequences from Pasteurellaceae–other sequences; yellow, conserved residues; blue and black letters, mismatches. The sequences have been aligned to nucleotides 181 through 299 of reference strain AY314034. The reference strain and nucleotide position of the reference strain are indicated by arrows. Mismatches between rpoB sequences from Pasteurellaceae species and biotypes are evenly distributed, thereby complicating development of a P. pneumotropica-specific PCR-based diagnostic assay using this gene.