Figure 6.

Detection of decreasing amounts of P. pneumotropica Heyl DNA in backgrounds of Pasteurellaceae–other and nonPasteurellaceae DNA by using real-time PCR assays. The real-time PCR assay for Heyl was run in triplicate wells in presence of various background DNAs. At a dilution factor of 0.01, Heyl DNA (approximately 10,000 copies) could not be detected in a background of Pasteurellaceae–other (Ct = undetermined compared with 20 for the other 3 groups). However, Heyl DNA could be detected in water or a background of nonPasteurellaceae DNA (Proteus mirabilis and Pseudomonas aeruginosa), even at a dilution of 10⁻⁵ (approximately 10 copies; Ct = 30).