Fig. 4.

Western blot analysis of cytoplasmic protein from E17.5 heads of Casp3 ^tm1Flv/Casp3 ^tm1Flv, Casp3 ^mldy/Casp3 ^tm1Flv, Casp3 ^tm1Flv/+, +/+, C3H, Casp3 ^mldy /Casp3 ^mldy, and Casp3 ^mldy /+ mice (n = 3). a Average protein expression levels from each of the genotypes with the standard error of the mean. Protein levels are normalised with α-tubulin as a loading control. An ANOVA was performed using Tukey HSD post hoc analysis. Significant differences in protein levels are shown: * reduced compared to C3H or +/+ (P ≤ 0.05); †, reduced compared to Casp3 ^mldy /+ (p ≤ 0.05); §, reduced compared to Casp3 ^mldy /Casp3 ^mldy (p ≤ 0.05); and ¥, reduced compared to Casp3 ^tm1Flv/+ (p ≤ 0.05). b An example Western blot probed with the Caspase-3 antibody and the α-tubulin loading control