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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: Toxicol Environ Chem. 2010 Oct;92(9):1673–1695. doi: 10.1080/02772241003711274

Figure 7. Effect of zinc on the expression levels of MT-3 in the MCF-10A cell line.

Figure 7

MCF-10A cells were cultured at a 1:10 ratio in the presence of MS-275 or AZC till they reached confluency, following which the cells were allowed to recover for 24 hr without the drug. The cells were then exposed to 100 μM Zinc for 24 or 48 hr. Total RNA was extracted from the cells and the expression of MT-3 was determined by real time PCR. The level of MT-3 expression was normalized to that of β-actin. The determinations were performed in triplicate and the results shown are the mean ± SEM. All treatment groups were statistically significant over untreated controls (p < 0.05). * denotes statistically significant from no zinc treated cells. # denotes statistically significant from no zinc treated cells.