Table 1. Locus name, clone name, GenBank accession number, primer sequence with fluorescent dye label forward (F) and reverse (R), clone repeat unit and primer annealing temperature (Ta°C) for D. tenebrosus microsatellite loci.
Locus | Clone name | Genbank accession number | Primer sequence (5′-3′) | Clone repeat unit | Ta°C |
Dicten02 | 020048 | GU187896 | F:(NED)ACCTGTGGACAGGAGGTTTG R:CATTCCCCTGTGTGGCTAAC | (GT)12 | 57 |
Dicten11 | 020320.2 | GU187905 | F:(FAM)CTCGACCACTTCGGTCAAAC R:TGCCTCTGGATACCTTGTGG | (CATA)6 | 57 |
Dicten18 | 020361 | GU187909 | F:(VIC)CGAATGGAGCACATACAGACC R:TTTCACAGGCTTTTGCAGTG | (ATAC)12 | 57 |
Dicten20 | 020363 | GU187910 | F:(NED)TCGCACTTTATAAATCCCAACAC R:ACCCAACCCCATAAGGTCTC | (TACA)18 | 57 |
Dicten25 | 020330 | GU187907 | F:(VIC)GCTTCTGCGGTGAAGGATG R:AGACCATCCGTAGCATGACC | (ATAC)14 | 57 |
Dicten27 | 020355 | GU187908 | F:(NED)AGGTTCGCGCTATATAAATCC R:GCACCACATGATGTTTGACAG | (ATAC)18 | 57 |
Dicten29 | 020326 | GU187906 | F:(VIC)TTCGTGATATATAACAACCAGCACR:CAAATCAGGCAAATACTTAATGG | (CATA)5 (CATG) (CATA)14 | 57 |