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. 2010 Dec 15;5(12):e14333. doi: 10.1371/journal.pone.0014333

Table 2. Summary of locus characteristics for D. tenebrosus by region (British Columbia, 12 populations pooled), North Cascades and South Cascades.

Region A Allele size range He Ho # Private alleles (frequency range) Null HW test
British Columbia (n = 291) # of streams not in HW (%)
Dicten02 5 168–190 0.374 0.450 4 (0.002–0.151) −0.241 0
Dicten11 4 139–149 0.504 0.825 2 (0.009–0.009) −0.444 11(91.7)
Dicten18 3 118–126 0.044 0.038 0 0.038 0
Dicten20 4 211–225 0.130 0.137 1 (0.002) −0.071 0
Dicten25 2 193–197 0.063 0.058 0 0.027 0
Dicten27 4 121–141 0.592 0.343 1 (0.138) 0.227 12 (100)
Dicten29 5 158–174 0.332 0.395 1 (0.010) −0.157 1 (8.3)
North Cascades (n = 22) P-value for HW test
Dicten02 2 178–188 0.416 0.59 1 (0.295) −0.360 0.121
Dicten11 2 139–147 0.268 0.32 0 −0.216 1.0
Dicten18 2 118–126 0.087 0.09 0 −0.080 1.0
Dicten20 3 203–231 0.606 0.50 0 0.138 0.101
Dicten25 1 193 0.000 0.00 0 0 -
Dicten27 3 133–141 0.492 0.36 0 0.175 0.07
Dicten29 2 158–182 0.087 0.00 0 0.238 0.024*
South Cascades (n = 9)
Dicten02 2 180–188 0.444 0.444 1 (0.667) 0 1.0
Dicten11 2 139–147 0.222 0.198 0 −0.118 1.0
Dicten18 2 122–126 0.111 0.105 0 −0.057 0.860
Dicten20 5 203–231 0.667 0.710 2 (0.056–0.111) 0.053 0.403
Dicten25 2 193–197 0.222 0.198 0 −0.118 1
Dicten27 1 133 0.000 0.000 0 0 -
Dicten29 5 158–182 0.444 0.457 1 (0.056) 0.044 0.548

n =  sample size of individuals, A =  number of alleles, He  =  expected heterozygosity, Ho  =  observed heterozygosity, Null  =  estimated null allele frequency (Oosterhout method), and the number of private alleles per locus and their frequency range for each region. Results of Hardy-Weinberg (HW) equilibrium tests are presented for British Columbia as the number of populations with significant deviation from HW expectations (P<0.05 after Bonferroni correction). Within the North Cascades and the South Cascades, P-values of HW tests are presented for each locus.

* =  significant deviation from HW equilibrium after Bonferroni correction.

 =  evidence for null alleles at this locus.

See Table S1 for population-level locus characteristics in British Columbia.