Table 2. Summary of locus characteristics for D. tenebrosus by region (British Columbia, 12 populations pooled), North Cascades and South Cascades.
Region | A | Allele size range | He | Ho | # Private alleles (frequency range) | Null | HW test |
British Columbia (n = 291) | # of streams not in HW (%) | ||||||
Dicten02 | 5 | 168–190 | 0.374 | 0.450 | 4 (0.002–0.151) | −0.241 | 0 |
Dicten11 | 4 | 139–149 | 0.504 | 0.825 | 2 (0.009–0.009) | −0.444 | 11(91.7) |
Dicten18 | 3 | 118–126 | 0.044 | 0.038 | 0 | 0.038 | 0 |
Dicten20 | 4 | 211–225 | 0.130 | 0.137 | 1 (0.002) | −0.071 | 0 |
Dicten25 | 2 | 193–197 | 0.063 | 0.058 | 0 | 0.027 | 0 |
Dicten27 | 4 | 121–141 | 0.592 | 0.343 | 1 (0.138) | 0.227† | 12 (100) |
Dicten29 | 5 | 158–174 | 0.332 | 0.395 | 1 (0.010) | −0.157 | 1 (8.3) |
North Cascades (n = 22) | P-value for HW test | ||||||
Dicten02 | 2 | 178–188 | 0.416 | 0.59 | 1 (0.295) | −0.360 | 0.121 |
Dicten11 | 2 | 139–147 | 0.268 | 0.32 | 0 | −0.216 | 1.0 |
Dicten18 | 2 | 118–126 | 0.087 | 0.09 | 0 | −0.080 | 1.0 |
Dicten20 | 3 | 203–231 | 0.606 | 0.50 | 0 | 0.138 | 0.101 |
Dicten25 | 1 | 193 | 0.000 | 0.00 | 0 | 0 | - |
Dicten27 | 3 | 133–141 | 0.492 | 0.36 | 0 | 0.175 | 0.07 |
Dicten29 | 2 | 158–182 | 0.087 | 0.00 | 0 | 0.238 | 0.024* |
South Cascades (n = 9) | |||||||
Dicten02 | 2 | 180–188 | 0.444 | 0.444 | 1 (0.667) | 0 | 1.0 |
Dicten11 | 2 | 139–147 | 0.222 | 0.198 | 0 | −0.118 | 1.0 |
Dicten18 | 2 | 122–126 | 0.111 | 0.105 | 0 | −0.057 | 0.860 |
Dicten20 | 5 | 203–231 | 0.667 | 0.710 | 2 (0.056–0.111) | 0.053 | 0.403 |
Dicten25 | 2 | 193–197 | 0.222 | 0.198 | 0 | −0.118 | 1 |
Dicten27 | 1 | 133 | 0.000 | 0.000 | 0 | 0 | - |
Dicten29 | 5 | 158–182 | 0.444 | 0.457 | 1 (0.056) | 0.044 | 0.548 |
n = sample size of individuals, A = number of alleles, He = expected heterozygosity, Ho = observed heterozygosity, Null = estimated null allele frequency (Oosterhout method), and the number of private alleles per locus and their frequency range for each region. Results of Hardy-Weinberg (HW) equilibrium tests are presented for British Columbia as the number of populations with significant deviation from HW expectations (P<0.05 after Bonferroni correction). Within the North Cascades and the South Cascades, P-values of HW tests are presented for each locus.
* = significant deviation from HW equilibrium after Bonferroni correction.
= evidence for null alleles at this locus.
See Table S1 for population-level locus characteristics in British Columbia.