Figure 2. rdx¹¹ is a null mutant.

(A) Classical mutant/deficiency tests were performed. The rdx¹¹/Df(3R)gamma7 heterozygotes were embryonic lethal, like the rdx¹¹/rdx¹¹ and Df(3R)gamma7/Df(3R)gamma7 homozygotes, suggesting that rdx¹¹ is a null allele. (B) Clones of rdx¹¹ mutant cells (GFP-negative) (a) in eye imaginal disc are superimposed with Ci-155 immunostaining (magenta) (b). Similar accumulation of Ci-155 was also observed in the clones of hib^Δ6, which was a null mutant of rdx isolated by Jiang's group, and also in the clones of Df(3R)Exel6171, a deficiency line (Zhang et al., 2006). These results also support the notion that rdx¹¹ is a null mutant.